FIG. 3.

Structure of genomic segments 1 to 3 of the 9B21 reovirus. (A) Ethidium bromide-stained 1% agarose gel with dsRNA purified from C. parasitica strain 9B21. (B) Northern blots of individual lanes of a 1% agarose gel containing 9B21 dsRNA, blotted and probed with digoxigenin-labeled PCR products representing segment 1 (nucleotides [nt] 2256 to 2988), segment 2 (nt 261 to 920), and segment 3 (nt 1728 to 2523). (C) Overall organization based on sequences from randomly primed clones made from gel-separated genome segments and 5′ RACE clones made for each terminus, as described in the text. The terminal 15 residues of the coding strand of each segment are shown.