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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Aging Cell. 2012 Dec 25;12(1):156–166. doi: 10.1111/acel.12032

Figure 6. Deletion of AFG3 reduces cytoplasmic translation and extends life span by a Sir2-independent, Gcn4-dependent mechanism.

Figure 6

(a) Representative polysome profiles of log phase wild type BY4742 yeast and afg3Δ mutant yeast grown in YPD at 30°C. Deletion of AFG3 causes a shift of ribosomes from highly translated mRNA to unbound forms. Curves are normalized by the minima between 80S free subunits and 2R disomes.

(b) Quantitation of peak areas from triplicate polysomes. *p<0.05, ns= not significant, error bars are s.e.m.

(c–f) Replicative lifespan curves of the indicated strains. Parentheses denote mean lifespan. afg3Δ requires Gcn4 for lifespan extension but does not require Sir2 or Fob1. afg3Δ is not additive with rpl20bΔ nor DR (0.05% glucose) for lifespan extension.

(g) Flow cytometric analysis of log phase genomically tagged Gcn4-GFP strains.