Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction of expression vector. (a) PCR products from three-round amplification. M, DNA marker; lane 1, GAPDH as internal control; lane 2, VH; lane 3, VL; lane 4, CH1; lane 5, CL; lane 6, heavy chain Fd; lane 7, light chain; lane 8, Fab. (b) Recombinant vector pComb3X/LF8-Fab recut by Sfi I. M, DNA marker; lane 1, pComb3XSS cut by Sfi I; lane 2, pComb3X/LF8-Fab recut by Sfi I; lane 3, PCR product of Fab.
