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Bioluminescence imaging of orthotopic tumour growth. Mice were orthotopically implanted on the caecum with DLD1-1 colorectal cancer cells engineered to express firefly luciferase (under control of the Simian Virus-40 promoter). At weekly intervals, the mice were injected with D-luciferin and imaged using an IVIS-50 system (Caliper Life Sciences, Runcorn, UK). The image shown depicts the growth of the orthotopic tumour over time.
