Figure 3.

URB597-mediated regulation of TH promoter activity is CB₁ cannabinoid receptor- and FAAH-independent. (A) Luciferase activity determined on cells transfected with pTH250-Luc and treated with AEA at 10 μM in the presence or the absence of SR 141716A (1 μM) and/or URB597 (0.1 μM). One-way ANOVA followed by Tukey's post-test, ***P < 0.001, **P < 0.01, *P < 0.05, relative to control; and ^##P < 0.01, ^#P < 0.05, as indicated between treated cells. (B) illustrates the influence of SR 141716A (1 μM) on the regulation of TH promoter activity mediated by URB597 or HU 210 (both at 0.1 μM). Two-way ANOVA indicates a general effect of SR 141716A (**P = 0.024, f = 10.26, residual d.f. = 48) with a positive interaction on the treatment (P = 0.0001, f = 11.16). ^###P < 0.001 as indicated between treated cells, determined with Bonferroni post-test. The responses induced by URB597 (0.1 μM) and other FAAH inhibitors (all at 10 μM) were compared to investigate the involvement of FAAH (C). One-way ANOVA indicated a global treatment effect (P = 0.0246, f = 3.396, residual d.f. = 18) with **P < 0.01 relative to control, given by Dunnett's post-test. All the results are given as the percentages of relative luciferase activity (firefly luciferase relative to Renilla liciferase) relative to control values. Data shown are means with SEM values of three to six experiments performed in triplicate.