Figure 5.

Effects of AngII treatment on CBP to NFκB and CREB binding in neuronal cells. The serum-starved (24 h) CATH.a cells were treated without or with AngII (100 nM; 6 h). (A) Densitometric analysis and a representative immunoblot showing increased expression levels of p-p65(Ser-276) and decreased p-CREB(Ser-133) in AngII-treated cells when compared to vehicle. (B) Densitometric analysis and a representative immunoblot showing increased CBP to p-p65(Ser-276) binding and decreased CBP to p-CREB(Ser-133) binding in AngII stimulated cells as measured by immunoprecipitation analysis. The results are means ± SD of three independent experiments. **P < 0.01; **P < 0.01, compared with cells treated with vehicle.