Skip to main content
. 2013 May 27;169(4):860–874. doi: 10.1111/bph.12177

Figure 6.

Figure 6

Inhibitory effects of L-sh-GSK3β on AngII-induced imbalance between PIC and AIC in neuronal cells. Serum-starved CATH.a cells were transduced with L-sh-GSK3β at a multiplicity of infection (MOI) of 30 for 48 h and were stimulated with 100 nM AngII for 6 h. (A) mRNA expression of TNF-α, IL-1β and IL-10. (B) Densitometric analysis and a representative immunoblot showing protein expression of TNF-α, IL-1β and IL-10. Pretreatment with L-sh-GSK3β resulted in reversal of AngII-induced increase in TNF-α and IL-1β, and attenuation in IL-10 levels indicating improved balance between PIC and AIC by GSK-3β inhibition. Cells transduced with scrambled sequences separately in presence of AngII did not show any effect. The results are means ± SD of three independent experiments. *P < 0.05 AngII versus vehicle-treated cells; #P < 0.05 AngII versus L-sh-GSK3β + AngII-treated cells; $P < 0.05 L-scrambled + AngII versus vehicle-treated cells.