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. 2013 May 27;169(4):860–874. doi: 10.1111/bph.12177

Figure 7.

Figure 7

Effects of GSK-3β knock down by lentiviral shRNA (L-sh-GSK3β) on AngII induced alterations in transcription factors in neuronal cells. Serum starved CATH.a cells were transduced with L-sh-GSK3β at a multiplicity of infection (MOI) of 30 for 48 h and were stimulated with 100 nM AngII for 6 h. Cell extracts were then immunoprecipitated with CBP and immunoblotted either with p-p65(Ser-276) or p-CREB(Ser-133). An immunoblot (upper panel) and densitometric analysis (lower panel) showing reversal of AngII-induced increased CBP to p-p65(Ser-276) and reduced CBP to p-CREB(Ser-133) binding in neuronal cells. Cells were also transduced with scrambled sequences in presence of AngII did not show any effect. The results are means ± SD of three independent experiments. ***P < 0.001.