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Primer sequences used for PCR and amplified product sizes
| Gene | Primer | Sequence (5′ to 3′) | Size (bp) |
|---|---|---|---|
| GAPDH | Forward | TGAATGACATCAAGAAGGTGGTGGAG | 239 |
| Reverse | TCCTTGGAGGCCATGTAGGCCAT | ||
| IL-1β | Forward | CTGTGTCTTTCCCGTGGACC | 200 |
| Reverse | CAGCTCATATGGGTCCGACA | ||
| IL-10 | Forward | CCAGTTTTACCTGGTAGAAGTGATG | 324 |
| Reverse | TGTCTAGGTCCTGGAGTCCAGCAGACTC | ||
| TNF-α | Forward | CCTCCCTCTCATCAGTTCTA | 501 |
| Reverse | GCAATGACTCTAAAGTAGACCTG |
