Table 2.
Mean diametersa of microbial growth inhibition zones (mm) of test samplesb
Microoganisms |
Samples |
|||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
EO |
F1 |
F3 |
F4 |
F5 |
Referencec |
|||||||||||
Gram (−) bacteria | 100% | 10% | 1% | 10% | 1% | 0.1% | 10% | 1% | 0.1% | 10% | 1% | 0.1% | 10% | 1% | 0.1% | 10 μg |
Escherichia coli ATCC 11775 |
12 |
9 |
6 |
6 |
6 |
6 |
9 |
8 |
6 |
8 |
8 |
7 |
9 |
7 |
7 |
21 |
Klebsiella pneumoniae ATCC 13883 |
11 |
8 |
6 |
8 |
7 |
6 |
8 |
6 |
6 |
9 |
8 |
7 |
8 |
6 |
6 |
20 |
Proteus mirabilis |
11 |
9 |
6 |
13 |
12 |
11 |
8 |
8 |
6 |
12 |
11 |
10 |
12 |
10 |
9 |
18 |
Pseudomonas aeruginosa ATCC 27853 |
13 |
9 |
6 |
9 |
7 |
6 |
9 |
7 |
6 |
7 |
6 |
6 |
7 |
6 |
6 |
24 |
Salmonella typhi ATCC 6539 |
11 |
9 |
6 |
8 |
8 |
6 |
8 |
7 |
6 |
7 |
7 |
6 |
6 |
6 |
6 |
24 |
Shigella flexneri |
18 |
13 |
8 |
12 |
8 |
6 |
10 |
8 |
6 |
9 |
7 |
6 |
8 |
7 |
6 |
23 |
Gram (+) bacteria |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Enterococcus faecalis ATCC 10541 |
16 |
12 |
6 |
12 |
10 |
9 |
10 |
8 |
6 |
10 |
8 |
8 |
13 |
11 |
10 |
20 |
Staphylococcus aureus ATCC 25922 |
16 |
11 |
7 |
10 |
8 |
6 |
11 |
8 |
6 |
9 |
7 |
6 |
8 |
7 |
6 |
25 |
Fungi |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Candida albicans ATCC 9002 |
13 |
7 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
22 |
Candida glabrata CIPA 35 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
23 |
Candida krusei ATCC 6258 |
10 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
18 |
Candida lusitaniae ATCC 200950 |
13 |
10 |
6 |
12 |
9 |
9 |
11 |
9 |
7 |
12 |
10 |
6 |
14 |
12 |
10 |
33 |
Candida parapsilosis ATCC 22019 |
7 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
6 |
24 |
Candida tropicalis ATCC 750 | 14 | 7 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 7 | 6 | 6 | 23 |
The results are the mean values of triplicate tests measured after 24 hrs (at 37°C for bacteria) and 48 hrs (at 35°C for fungi) incubation; aDiameter value of 6 mm is equal to disc diameter, implying no activity; bEssential oil (EO), fractions of essential oil (F1, F3, F4 and F5); cReference compounds were gentamicin and nystatine for bacteria and fungi respectively.