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. 2013 Apr 3;99(1):175–184. doi: 10.1093/cvr/cvt083

Figure 3.

Figure 3

TGF-β1 mRNA expression and p-SMAD2/3 levels in mitral valves from SOD1 knockout mice compared with wild-type littermates. (A) TGF-β1 expression was unaltered in SOD1-deficient mitral valves (n= 9 wild-type valves, n= 13 knockout valves). (B–E) Immunoblots showing SMAD2/3 phosphorylation (B and D) and subsequent quantitation using densitometry (C and E). Note that similar to TGF-β1 expression, SMAD2/3 phosphorylation is not changed in mitral valves with SOD1 deficiency (n= 4 wild-type valves, n= 4 knockout valves). (F–H) Pro-fibrotic and matrix remodelling gene expression in mitral valves from wild-type and SOD1-deficient mice. Note that CTGF and MMP-2 are significantly increased in SOD1 knockout mice (n= 9 wild-type valves, n= 13 knockout valves). *P < 0.05 in all figures.