Fig. 3.
Fluorescence spectra of FRET probes in the presence and absence of a complementary DNA template. PNA-Cy5 and FAM-PNA were incubated at the concentrations indicated (μM) in 100 mM Tris 5 mM MgCl2, pH 7.1. in the presence or absence of a complementary DNA and excited at 488 nm. FAM-PNA •DNA and PNA-Cy5•DNA were used as controls for the individual PNAs bound to DNA.