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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Org Biomol Chem. 2013 Mar 28;11(19):3159–3167. doi: 10.1039/c3ob26923j

Fig. 4.

Fig. 4

Fluorescence spectra of FRET PNA probes in the presence or absence of in vitro transcribed iNOS mRNA. A) 0.2μM FAM-PNA and PNA-Cy5 or mmPNA-Cy5 (mismatched) were incubated with 0.2 μM in vitro transcribed iNOS mRNA at 37°C for 30 min in Opti-MEM® medium and excited at 488 nm. The PNAs were also heated with iNOS mRNA to 65°C for 1 min before incubation at 37°C for 30 min. B) 0.2 μM FAM-PNA and PNA-Cy5 were annealed to their partially complementary ODNs, and incubated with the iNOS mRNA at 37 °C for 30 min. As controls, 0.2μMFAM-PNA (0.2 μM) and PNA-Cy5 (0.2 μM) were separately incubated with iNOS mRNA under the same conditions.