FIG. 2.

Proinflammatory cytokine and chemokine production in MMTV-stimulated BMDCs. Cells (10⁶/100 μl) were incubated with LPS (100 ng/ml) or MMTV. Where indicated, LPS and MMTV were boiled (1 h) or pretreated with UV (30 min) or anti-MMTV antibody (Ab). (A) After 18 h, the concentration of TNF-α, IL-6, or IL-12 in the supernatant was measured by ELISA. The data are presented as means plus SD. One experiment out of three is shown. *, P < 0.001 compared to the control group (unstimulated BMDCs). (B) After 2 h, cytokine and chemokine mRNA levels were measured by RPA, using multiprobe sets. One experiment out of three is shown.