Fig. 2.

The mTOR inhibitor rapamycin promotes infection and killing by MYXV in half of the BTICs in vitro. (A) Cell viability assay (Alamar Blue) treated with MYXV (MOI = 1) + rapamycin (100 nM) in BTICs (5 days p.i). (B) Self-renewal of BTIC lines after pretreatment with rapamycin (100 nM) followed by MYXV infection. BTICs were infected with MYXV (MOI = 1); 5 days after infection they were replated and then after 10 days the spheres were counted. *P < .05 compared by Student's t-test with the MYXV alone. (C) Viral titers were obtained in BTIC lines after MYXV (MOI = 1) + rapamycin (100 nM) at 72 h p.i. Values represent mean FFUs ± SD from triplicate wells. **P < .01 compared by Student's t-test with the MYXV alone.