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. 2013 Apr 12;15(7):904–920. doi: 10.1093/neuonc/not035

Fig. 7.

Fig. 7.

Fig. 7.

Promotion of MYXV infection in human BTICs by rapamycin is independent of type-I IFN and dependent on the viral MT5 gene in half of the BTICs. (A) Real-time PCR of BTIC lines 24 h post-stimulation (CT, control; IFN, 10 U/mL IFN-β; PIC, 25 μg/mL polyI:C; MV, 1.0 MOI MYXV; VSV, 1.0 MOI of delta51–vesicular stomatitis virus) for type-I IFN and type-I IFN stimulated genes. (B) Absorbance readings of HEK-Blue Assay (Invitrogen) measuring secreted type-I IFN from BTICs 24 h posttreatment (n = 4). Error bars indicate standard error. (C) Quantified GFP expression after infection with 1.0 MOI wild-type MYXV or vMyxMT5KO at 72 h p.i. (D) Representative viral titers on BTICs and glioma cell lines after infection with MYXV and vMyxMT5KO at 72 h p.i.