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Mutations introduced in the TGEV full-length cDNA and virus recovery. Nucleotide substitutions were introduced in the 3a gene CS (CS-B mutants [A]), the leader CS (CS-L mutants [B]), in both the CS-L and CS-B (double mutants [D]), and leader CS mutants with changes allowing non-Watson-Crick base pairing with the body cCS (non-Watson-Crick mutants [C]). Virus titers (PFU per milliliter) obtained for the passage 0 supernatant are indicated in the figure.
