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Reverse oligonucleotides used for RT-PCR analysis of RNA from rTGEV-infected cells
| sgmRNA | Primer | Sequence (5′→3′) | Amplicon size (bp) |
|---|---|---|---|
| Genomic | 1a-156-RS | TCCTTCGATCGCAATCAA | 473 |
| mRNA-S | S-449 | TAACCTGCACTCACTACCCC | 499 |
| mRNA-3a | 3a-169-RS | CAATAATGGAGAGACCAAG | 295 |
| mRNA-3b | X2B-112 | TTAACATACCAAAAGTATGC | 458 |
| mRNA-E | IGSM | CAGTCGACAGGCCTCGCCGGCGCGGCCGCGTTTAGTTCAAGC | 393 |
| mRNA-M | M.415RS | AGACCACCAAGAGTTAGTCC | 530 |
| mRNA-N | N-268RS | GGTCCGGTACCTAAGTAGTAGAAGAACC | 386 |
| mRNA-7 | 7(213)RS | TCTGTAGCAGCAAAATCC | 302 |
