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Oligonucleotides used for real-time RT-PCR analysis
| Amplicon | Size (bp) | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|---|
| Virus | 80 | TTCTTTTGACAAAACATACGGTGAA | CTAGGCAACTGGTTTGTAACATCTT |
| mRNA-3a.1 | 102 | CGGACACCAACTCGAACTAAACTTAC | ATCAAGTTCGTCAAGTACAGCATCTA |
| mRNA-3a.2 | 93 | CGTGGCTATATCTCTTCTTTTACTTTAACTAG | ATGGACGTGCACTTTTTCAATTG |
