TABLE 3.

Infectivities of Ebola VLPs possessing GPs of a different Ebola subtype, Marburg virus, or mutant GPs^a

GP	Infectious units (log10/ml)
Ebola Zaire GP mutants
GP I623A (mutant GP)	0.8 ± 0.3
GP L585A (mutant GP)	2.3 ± 0.4
Ebola virus
Zaire	2.3 ± 0.5
Reston	2.7 ± 0.4
Sudan	1.9 ± 0.2
Ivory Coast	2.3 ± 0.3
Marburg virus	2.7 ± 0.5

^a293T cells were transfected with plasmids for the expression of seven Ebola Zaire virus structural proteins and with p3E5EGFP and pC-T7Pol. Seventy-two hours later, the supernatant containing Ebola VLPs was harvested and incubated with fresh 293T cells transfected with plasmids expressing L, NP, VP35, and VP30. Seventy-two hours after infection, the number of GFP-expressing cells (corresponding to the number of infectious VLPs) was determined with a fluorescence microscope. Results are means ± standard deviations.