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. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: Cell. 2012 Jun 8;149(6):1269–1283. doi: 10.1016/j.cell.2012.04.026

Figure 5. p533KR Loses Its Ability to Induce Cellular Senescence in p53 3KR/3KR MEFs.

Figure 5

(A and B) qRT-PCR analysis of indicated mRNAs in p53+/+, p533KR/3KR and p53−/− MEFs either untreated or treated with 0.2 μg/ml Dox for 8 hours. Results shown are the relative amount of specific mRNA normalized first to β-actin and then to the untreated wildtype sample values from three independent experiments with different MEF lines. Error bars represent ± SEM.

(C) Cell growth rate analysis of p53+/+, p533KR/3KR and p53−/− MEFs. 3 × 104 MEFs of different genotypes were seeded into 6-well plates at day 0 and counted daily.

(D) Images of SA-β-gal staining of p53+/+ and p533KR/3KR MEFs at different passages cultured according to the 3T3 protocol. MEFs at indicated passages were fixed and stained for β-galactosidase activity.

(E and F) Immunoblot analysis of p53, Arf and p21 in p533KR/3KR (E) and p53−/− (F) MEFs at indicated passages cultured according to the 3T3 protocol. β-actin serves as a loading control.

See also Figure S7.