FIG. 6.
Involvement of L-domain function in enhanced EIAV budding induced cytoD treatment. (A) Equal amounts of ED cells chronically infected with EIAVuk proviruses containing specific L domains (EIAV YPDL, HIV-1 PTAP, or RSV PPPY) (37) were incubated with the indicated agents at 37°C for 2.5 h. Virion production was examined by measuring the RT activity in culture medium after treatment. (B) Equal amounts of L-domain-positive and -negative producer cells were incubated with cytoD at the indicated concentrations for 2.5 h at 37°C, and viral particles in the culture medium were then collected by centrifugation. The pelleted virions were resuspended in 1× phosphate-buffered saline. The RT activity associated with the resuspended virions was tested as a measure of EIAV production. The data represent the mean value with standard deviation of duplicate experiments. The astrisks indicate a statistically significant difference (P < 0.05) compared to the untreated control.