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. 2004 Jan;78(2):790–797. doi: 10.1128/JVI.78.2.790-797.2004

FIG. 2.

FIG. 2.

(A) Nucleotide and amino acid sequence of NS-3 protein showing (i) the two first ATG codons and the TAA stop codon (boldface italic characters) mutated to generate pJNS3mATG1, pJNS3mATG2, pJNS3mATG1+2, and pJΔNS3 constructs, respectively; (ii) the two NsiI restriction sites used to generate pJNS3ΔNsiI construct; (iii) the oligopeptidic sequence used to prepare a specific anti-NS3 antiserum (boldface characters); (iv) putative zinc-finger motifs; (v) N-glycosylation sites (NXT/NXS); and (vi) phosphorylation site (RRYS) (underlined). (B) Alignment of NS-3 sequences of JcDNV (the present study), MlDNV(Fédière, unpublished), GmDNV (GenBank accession number NC_004286), and DsDNV (GenBank accession number NC_001899) by using the Multalin program (8).