FIG. 4.

(A) Infectivity tests of pJNS3ΔNsiI, pJΔNS3, pJNS3mATG1, pJNS3mATG1+ATG2, pJNS3mATG2, and pBRJ constructs transfected to fourth-instar S. littoralis larvae. Mock, mock-transfected larvae. Purified plasmid DNA (10 μg/larva) was injected with DEAE-dextran transfection reagent. At 15 days posttransfection, virus infection was detected in larval-pupal homogenates by an ELISA. Error bars indicate the standard errors of the means of at least three experiments. (B) Infectivity tests of virions produced in Ld 652-transfected cells. Five days posttransfection with the different constructs, cells were homogenized, and cell extracts were inoculated to fourth-instar S. littoralis larvae. Fifteen days later, virus infection was detected in larval-pupal homogenates by ELISA. Error bars indicate the standard errors of the means of at least three experiments.