Pharmacological profiles of vesicular [3H]DA uptake in rat striatum in the presence of Tat1–86, Tat Cys22, or tetrabenazine (TBZ, a VMAT2 inhibitor). Striatal synaptosomes were preincubated with various concentrations of Tat1–86, Tat Cys22, or TBZ (0.1 nM–10 µM) at 34°C for 15 min followed by the addition of [3H]DA (final concentration, 0.1 µM) for 8 min. Tat Cys22 and TBZ were used as negative and positive controls, respectively. Data are expressed as mean ± S.E.M. as percentage of control (CON) values (28,205 ± 1965 dpm) from five independent experiments performed in duplicate. Nonspecific [3H]DA uptake was determined in the presence of 10 µM nomifensine. All curves were best fit to a single class of binding site and generated by nonlinear regression.