Table 3.

Association between smoking status (never, current, former) and polygenic risk score with bladder cancer risk among white males in US studies¹

	Cases	Controls	OR²	95% CI)
Smoking status
Never	254	464	Ref.
Ever	1130	882	2.57	(2.03,3.15)
Former	849	704	2.23	(1.74,2.85)
Current	264	156	4.79	(3.43,6.70)
Polygenic risk score (12 SNPs)
Score 0 (low risk)	171	325	Ref.
Score 1	326	343	1.87	(1.46,2.39)
Score 2	392	346	2.22	(1.74,2.82)
Score 3 (high risk)	495	332	2.94	(2.32,3.73)

Smoking status association by polygenic risk score (12 SNPs)³
Ever vs Never	never	ever	never	ever
Score 0 (low risk)	37	134	98	227	1.77	(1.11,2.84)
Score 1	61	265	117	226	2.50	(1.68,3.70)
Score 2	72	320	129	217	2.82	(1.94,4.11)
Score 3 (high risk)	84	411	120	212	3.11	(2.17,4.45)
			Test for interaction ⁴= 0.235
Former vs Never	never	former	never	former
Score 0 (low risk)	37	98	98	174	1.55	(0.95,2.53)
Score 1	61	197	117	185	2.12	(1.40,3.19)
Score 2	72	249	129	174	2.49	(1.69,3.68)
Score 3 (high risk)	84	305	120	171	2.66	(1.83,3.87)
			Test for interaction⁴= 0.272
Current vs Never	never	current	never	current
Score 0 (low risk)	37	34	98	47	3.31	(1.74,6.27)
Score 1	61	66	117	35	5.29	(3.01,9.29)
Score 2	72	65	129	38	4.66	(2.69,8.08)
Score 3 (high risk)	84	99	120	36	6.15	(3.64,10.42)
			Test for interaction⁴= 0.373

Polygenic risk score (PRS) calculated as quartiles of a linear predictor including 12 SNPs in table 2. The PRS is defined only for subjects with complete data on all 12 SNPs.

OR from logistic regression models adjusted for study, age, and gender

Median (range) number of risk alleles for each genetic score in the control population is: 10 (5-14) for score 0; 12 (9-17) for score 1; 13 (11-16) for score 2; and 15 (13-20) for score 3 (the maximum number of risk alleles is 20 because rs11892031 and rs17863783 are considered as dichotomous variables due to the low frequency of the rare homozygous genotypes).

⁴

LRT (3df)