Table 2. Estimated processing times.

Tests have been performed on the example movie using Ubuntu 10.04, 64 bit 3,2Ghz 4 Cores and Matlab R2011a. The movie used for benchmarking the software is a single channel FSM movie with 91 frames of 656 by 1118 pixels.

Step	Processing time	Notes
Noise estimation	3 s	Region of interest: 300×650 pixels.
Thresholding	15 s	Default parameters
Mask Refinement	7 s	Default parameters
Speckle detection	2 min	Maximum number of iterations: 3 Total number of speckles: ~2500/frame
Flow tracking	5 hr 30 min	Template size: 11 pixels Average time: 0.3 s/per speckle per frame
Speckle tracking	5min	Gaussian weighted interpolation
Kinetic analysis	8 min
Flow analysis	10 min	Number of frames for time averaging: 15