a. Mean free asparagine concentration (mmol kg−1) in grain of five commercially used rye varieties grown in 2009–2010 (n = 18). SED = 0.379 on 4 degrees of freedom (df). The least significant difference (LSD) at 5% = 1.051, and is indicated. b. Mean total free amino acid concentration (mmol kg−1) in grain of five rye varieties grown in 2009–2010 (n = 18). SED = 0.659 on 4 df. LSD (5%) = 1.830 c. Mean asparagine concentration (mmol kg−1) in grain of rye grown under different nitrogen fertilisation regimes (1, 100, 200 kg ha−1) (n = 30). SED = 0.428 on 10 df. LSD (5%) = 0.953. d. Mean total free amino acid concentration (mmol kg−1) in grain of rye grown under different nitrogen fertilisation regimes (1, 100, 200 kg ha−1) (n = 30). SED = 1.02 on 10 df. LSD (5%) = 2.272.