FIG. 2.
TSG-5′ inhibits EIAV/PTAP, but not EIAV/WT or EIAV/PPPY, VLP release. 293T cells were transfected with vectors expressing EIAV/WT, EIAV/PPPY, or EIAV/PTAP Gag alone (lanes 1, 3, and 5) or were cotransfected with Gag vectors and the TSG-5′ expression vector at a 1:1 DNA ratio (lanes 2, 4, and 6). (A) Cell and viral lysates were radioimmunoprecipitated with horse anti-EIAV antiserum. (B) The cell lysates were immunoblotted with anti-HA antiserum to detect TSG-5′ expression. For panels A and B, the positions of the EIAV Gag precursor (Pr55Gag) and TSG-5′ are indicated on the left, and the positions of molecular mass markers are shown on the right. (C) Virus release efficiency, calculated as described in the legend to Fig. 1. The data are averages from at least three independent experiments plus or minus the standard error.