FIG. 4.

EIAV/PTAP and EIAV/PPPY, but not EIAV/WT, VLP release is inhibited by TSG-F and TSG-3′. 293T cells were transfected with vectors expressing EIAV/WT, EIAV/PPPY, or EIAV/PTAP Gag alone (lanes 1) or were cotransfected with Gag plasmids and vectors encoding TSG-3′ (A) or TSG-F (B) at 1:1 DNA ratios (lanes 2). Cell and viral lysates were radioimmunoprecipitated with horse anti-EIAV antiserum, and the cell lysates were subjected to immunoblotting with anti-HA antiserum for the detection of TSG-3′ and TSG-F. Virus release efficiency was calculated as described in the legend to Fig. 1. The data are averages from at least three independent experiments plus or minus the standard error. Molecular mass markers are indicated on the right.