Fig. 1.

The structure of the T-DNA region in the binary vectors of p13AWY-1 (A) and pCAMBIA1301 (B). Waxy-pro and Int1: the promoter (1288 bp) and first intron (1116 bp) of the Waxy gene from rice cv. 232; Anti-Wx: an antisense DNA fragment (756 bp) of the Waxy gene from rice cv. 232; hpt: the hygromycin phosphotransferase gene; gus: the β-glucuronidase (GUS) gene; 35S-pro and 35S-ter: the 35S promoter and terminator of CaMV; Nos-ter: the terminator of the nos synthase gene; LB and RB: the left and right border of the T-DNA; Xh: XhoI; B: BamHI; E: EcoRI; Bs: BstXI; Sa: SacI; K: KpnI; S: SmaI; X: XbaI; Sal: SacI; P: PstI and H: HindIII. The arrows show the sizes and locations of the PCR products.