Table 4.
Marker | Tm (°C) | Forward primer (5′→3′) | Reverse primer (5′→3′) |
---|---|---|---|
cfa2155 | 60 | TTTGTTACAACCCAGGGGG | TTGTGTGGCGAAAGAAACAG |
cfa2141 | 60 | GAATGGAAGGCGGACATAGA | GCCTCCACAACAGCCATAAT |
cfa2185 | 60 | TTCTTCAGTTGTTTTGGGGG | TTTGGTCGACAAGCAAATCA |
wmc110 | 61 | GCAGATGAGTTGAGTTGGATTG | GTACTTGGAAACTGTGTTTGGG |
gwm126 | 60 | CACACGCTCCACCATGAC | GTTGAGTTGATGCGGGAGG |
gwm291 | 60 | CATCCCTACGCCACTCTGC | AATGGTATCTATTCCGACCCG |
barc308 | 55 | GCGATCTTGCGTGTGCGTAGGA | GCGTGGGATGCAAGTGAACAAT |
barc142 | 52 | CCGGTGAGAGGACTAAAA | GGCCTGTCAATTATGAGC |
wmc28 | 51 | ATCACGCATGTCTGCTATGTAT | ATTAGACCATGAAGACGTGTAT |