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. 2004 Jan;78(2):821–833. doi: 10.1128/JVI.78.2.821-833.2004

FIG. 1.

FIG. 1.

HPV16E1∧E4 is associated with reorganized keratin network in vivo and in vitro. (A and D) Tissue sections from cervical lesions caused by HPV16 were triple stained for 16E1∧E4 (green), K13 (red), and DNA (blue). The dotted lines indicate the position of the basal layer. The images were taken using a 10× objective. The cells enclosed within boxes are shown at higher magnification in panels B, C, and E (images captured using a 100× objective). (B) Colocalization of 16E1∧E4 and K13 filaments. The image corresponds to the right-hand box in panel A. (C) Association of reorganized keratin networks with 16E1∧E4. The image corresponds to the left-hand box in panel A. (E) Association of reorganized keratin networks with 16E1∧E4. The image corresponds to the left-hand box in panel D. The small box in the top right-hand corner of panel D, which contains cells with normal keratin staining, is shown enlarged in the inset. (F) 16E1∧E4-associated keratin network collapse in cultured epithelial cells is shown for comparison 24 h after infection of SiHa cells with rAd16E1∧E4. The cells were stained to show the presence of 16E1∧E4 (green), K18 (red), and DNA (blue). The image was taken using a 40× objective.