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. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: Ren Fail. 2010;32(9):1103–1108. doi: 10.3109/0886022X.2010.504912

Figure 2.

Figure 2

Effects of high-glucose PDS and glutathione on claudin-1 expression of HPMCs. (a) HPMCs treated with various media. Immunostaining of claudin-1 were detected after 24 h of incubation (×600). Claudin-1 decreased and became discontinuous after treated with 4.25% glucose PDS. Glutathione could reverse high-glucose-mediated reduction of claudin-1 expression. A, Control: DMEM; B, 1.5% glucose PDS; C, 4.25% glucose PDS; and D, glutathione + 4.25% PDS. (b) Western blot analysis of claudin-1 after HPMCs were cultured in different media. Claudin-1 expression was markedly reduced by 4.25% glucose PDS, whereas glutathione could obviously reverse the changes. Data show the mean values of three experiments. The control value was assumed to be 100%. *p < 0.05 versus control; **p < 0.05 versus 1.5% PDS group; and ***p < 0.05 versus 4.25% PDS group.