Figure 4. Gene expression analysis in dry and imbibed seeds.

Gene expression was investigated by qRT-PCR during the course of the imbibition process. Two-week stored seeds were used for mRNA extraction, and three replications were performed. Primers used in the qRT-PCR assay are listed in Table S1. (A) GA biosynthesis genes. (B) GA catabolism genes. (C) RGL3, a negative regulator of GA signaling. (D) ABA biosynthesis genes. (E) ABA catabolism genes.