Figure 4. Measurement of RNA editing extent by RNA-seq is accurate and highly reproducible.

(A) The editing extent of 226 organelle editing events, 106 mitochondrial :9 sites and 3 to 19 genotypes assayed per site and 120 plastid: 12 sites and 7 to 11 genotypes assayed per site, was measured by RNA-seq and PPE assay. A high correlation with PPE assay (R² = 0.97), the most precise method to measure editing extent, demonstrates the robustness of RNA-seq to evaluate organelle editing extent. (B) Two libraries with different indexes were prepared from the same sheared cDNA obtained from rip1 mutant. The editing extent measured between the two libraries shows a very high correlation (R²>0.99).