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Generation of deletion in cosmid clones. The figure shows an overview of the RARE cleavage procedure used to delete the 132-bp repeats in SN5 and A6. The short lines numbered 1 and 2 represent oligonucleotides 1 and 2, used to protect two TaqI sites. The two TaqI sites shown flank a 411-bp fragment of cosmid DNA that contains two copies of the 132-bp repeat.
