Figure 5. Knockdown of CG8093 and CG6277 in the midgut increases whole body TAG, starvation sensitivity in w¹¹¹⁸ and dcerk¹ flies and knockdown of CG6277 also leads to cardiac defects.

(A). Midgut specific reduction in CG8093 increases whole body TAG level to 325–350% in control w¹¹¹⁸ flies and about 275% in dcerk¹ mutants. The TAG level of driver is taken as 100%. n = 3, error bars represent standard deviation. (B). Midgut specific reduction in CG6277 increases whole animal TAG to 150% in control w¹¹¹⁸ flies and about 275%–300% in dcerk¹ mutants. The TAG level of driver is taken as 100%. n = 3, error bars represent standard deviation. (C). Reduction in CG8093 lipase increases the starvation sensitivity of wild type and dcerk¹ flies. CG8093 RNAi flies, esgGAL4 driver flies, 8093 RNAi combined to esgGAL4 in wild type and dcerk¹ are subjected to starvation and the number of surviving flies is recorded at 6 hr intervals. 200 flies divided into 10 groups for each genotype are used in one experiment. Three independent experiments are performed and the average percentage survival is shown for each time interval. (D). Reduction in CG6277 lipase increases the starvation sensitivity of wild type and dcerk¹ flies. CG6277 RNAi flies, esgGAL4 driver flies, 6277 RNAi combined to esgGAL4 in wild type and dcerk¹ are subjected to starvation and the number of surviving flies is recorded at 6 hr intervals. (E). Quantification of cardiac function parameters in RNAi knockdown of CG6277 in wild type and dcerk¹. Knockdown results in decreased heart rate in wild type flies and a similar trend in dcerk¹, increased heart period in both backgrounds, longer diastolic interval in control and mutant and no significant change in systolic interval. n = 11–13 for each group. Bar represents standard error of mean and statistical significance was determined using Student's t test; * denotes P< = 0.05-0.01, ** is P< = 0.01-0.001 is and *** denotes P< = 0.001-0.0001.