Figure 7. Activation of AKT, decrease in FOXO and activation of glycolytic genes in CERT heterozygous mice.

(A). Total AKT and phosphoAKT levels are monitored in liver extracts from wild type (+/+) and CERT heterozygous (+/−) mice. PhosphoAKT level is increased in CERT heterozygotes while total AKT is not significantly changed. (B). Comparison of different FOXO in liver tissue of wild type (+/+) and CERT heterozygous (+/−) mice shows significant decrease in their transcript levels. (C). Comparison of glycolytic genes in skeletal muscle from wild type (+/+) and CERT heterozygous (+/−) mice shows greater than 5 fold increase in transcript levels of phosphoglycerate mutase and pyruvate kinase and 1.8 fold increase in hexokinase. (D). A simple graphical model depicting metabolic reprogramming in dcerk¹ due to increased AKT activation and decreased FOXO to maintain energy balance. dcerk¹ mutants show glucose utilization through increased glycolytic flux mediated by Pglym and downstream enzymes. They also show increased utilization of triglycerides mediated by CG8093 and CG6277.