Figure 4. RNA-seq validation for ORFs newly identified by SILAC.

ORFs detected by SILAC-based MS were compared to deep sequencing data (RNA-seq). (A–H) Integrated Genome Browser (Affymetrix) screenshots depict gene loci with nucleotide positions referring to the plus-strand. RNA-seq data are visualized as numbers of cDNA reads per nucleotide (red plots). Grey and blue arrows represent annotated and new ORFs, respectively. For further details on new ORFs see Table S4.