Figure 2. Reactivity of chimpanzee plasma samples to VP1 proteins of chimpanzee polyomaviruses.

Antibody reactivity was assessed against the 4 chimpanzee polyomaviruses ChPyV, PtrovPyV3, PtrovPyV4 and PtrosPyV2 using plasma of 40 chimpanzees. Samples were analysed for seroreactivity with a capsomer-based IgG ELISA using the VP1 major capsid protein of the above polyomaviruses as antigens. The spread of absorbance measurement is shown with black dots, and cut-off values (COVs) are depicted with solid lines (PtrovPyV3: 0.028; PtrovPyV4: 0.023; PtrosPyV2: 0.013). A COV for ChPyV could not be calculated because all OD₄₅₀ values were >0.3.