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. 2013 Jun 20;9(6):e1003445. doi: 10.1371/journal.ppat.1003445

Figure 7. SBT3.3 expression promotes chromatin remodeling and is under epigenetic control.

Figure 7

(A) Comparative level of H3K4me3 and H3K9ac mark setting on the SBT3.3 gene promoter as present in leaf samples from Col-0, SBT3.3OEX1 and nrpd2 plants. Data are standardized for Col-0 histone modification levels. Data represent the mean ± SD; n = 3 biological replicates. (B) RT-qPCR of SBT3.3 transcript levels in Col-0 and nrpd2 plants. Data represent the mean ± SD; n = 3 biological replicates. Expression was normalized to the expression of the constitutive ACT2 gene and then to the expression in Col-0 plants. (C) RT-qPCR of SBT3.3 transcript levels in Col-0 seedlings upon treatment with 50 µM and 100 µM of sulfamethazine (SMZ) compared to mock (DMSO). Data represent the mean ± SD; n = 3 biological replicates. Expression was normalized to the expression of the constitutive ACT2 gene, then to mocked Col-0 plant expression. (D–G) Comparative level of H3K4me3 and H3K9ac mark setting on PR-1 (D), SBT3.3 (E), WRKY6 (F) and WRKY53 (G) gene promoters in Col0 and sbt3.3 plants following treatment with 100 µM BTH compared to buffer alone (mock). Data represent the mean ± SD; n = 3 biological replicates.