Table 2. Residual protease activity under different inhibitors.
Intestinal contents | No inhibitor (control) | Aprotinin (serine-protease inhibitor) | E64 (cysteine-protease inhibitor) | Pepstatin (aspartic-protease inhibitor) | EDTA (metalloprotease inhibitor) |
Crop | 100 | 45±4* | 125±18 | 123±7 | 117±3* |
Style sac | 100 | 38±3* | 99±5 | 108±5 | 106±4 |
Coiled gut | 100 | 84±9 | 100±8 | 98±7 | 78±6 |
Percent inhibition (mean ± SEM) was calculated as residual protease activity in each group exposed to an inhibitor, divided by protease activity in the control group (without inhibitor) and multiplied by 100. N was 9 in each group. Ninety nine % confidence intervals were used to assess the significance of differences (asterisks) from the control group.