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. 2013 Jun 20;8(6):e66892. doi: 10.1371/journal.pone.0066892

Figure 1. Over-expression of cJun/AP-1 results in increased PAK3 expression.

Figure 1

A & B: Real time RT-PCR analyses of PAK3 mRNA expression in Rat1a-GFP control cells and Rat1a-J4 cells grown in the absence and presence of doxycycline. Cells were grown in anchorage-dependant (A) and anchorage-independent (B) conditions. Results are the mean±S.E. of at least three independent experiments. (*p≤0.05) C & D: Western blot analysis showing PAK3 and cJun expression levels in anchorage-dependent (C) and anchorage-independent (D) growth conditions in Rat1a-J4 cells grown with or without doxycycline. Bar graphs show PAK3 protein levels, relative to the β-tubulin levels for each sample,±S.E.M over three independent experiments. (*p≤0.05) E: Western blot analysis showing PAK1 and PAK2 protein levels in response to doxycycline induction in anchorage-independently grown inducible Rat1a cells.