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. 2004 Jan;78(2):642–649. doi: 10.1128/JVI.78.2.642-649.2004

FIG. 2.

FIG. 2.

Schematic representation of HBV genome organization and the deletion variants. (A) HBV genome organization in the plasmid expressing HBV pgRNA. A black rectangle represents the HBV sequence (nt 1807 to 3182 and 1 to 1986), which is transcribed from the CMV immediate early promoter (dotted box) in the plasmid expressing HBV pgRNA. The positions of DR1 (nt 1833 to 1844) and DR2 (nt 1588 to 1598) are indicated. Locations of the ORFs for viral proteins are shown as white boxes. The plasmids expressing WT and variant pgRNAs did not express C, P, or envelope proteins. C and P proteins were provided by an expression plasmid during transfection (see Materials and Methods). (B) Representation of the locations of the HBV sequences deleted in the variants. The names of the variants indicate the first and last nucleotides of the HBV sequence being deleted (inclusive). On the right panel are the mean values and the standard deviations of the relative RC DNA proportion of each variant compared to that of the WT. The values are an average of measurements made from multiple independent transfections (at least five for HepG2 and at least six for Huh7).