Figure 1. PE-induced changes in the intracellular (A) and extracellular homocysteine concentrations (B), and changes in the intracellular Cu concentrations (C) in primary cultures of neonatal rat cardiomyocytes.

The cells were cultured in 10% FBS media for 24 h before medium change to serum-free media. The cells were then cultured for 72 h (Control), in the presence of 100 µM PE (PE), 1 µM Adox (Adox), or both PE and Adox (PE+Adox). The data were obtained from three independent experiments, and each experiment contains triplicate samples for each treatment. Values are means ± SEM. * or # significantly different from control group and # significantly different from PE-treated group (P<0.05).