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. 2013 Jun 20;8(6):e67510. doi: 10.1371/journal.pone.0067510

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© 2013 Vanhomwegen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A neighbor-joining tree was constructed based on the bont/A sequence retrieved by the RMA for the enrichment culture of the C. botulinum strain in a naturally contaminated salad specimen, and the corresponding sequences of 6 strains representative of 5 known C. botulinum toxinotype A subtypes: strain Hall (A1), NCTC 2916 (A[B]), Kyoto (A2), Loch Maree (A3), 657 (Ba4) and A661222 (A5). Bootstrap values and genetic distance (bar) are shown. The Genbank accession number of each strain is indicated in parentheses. The bont sequence obtained from the contaminated salad specimen is marked in bold.