. Author manuscript; available in PMC: 2014 Aug 15.

Published in final edited form as: J Comp Neurol. 2013 Aug 15;521(12):10.1002/cne.23320. doi: 10.1002/cne.23320

Table 1.

Primer sequences, 5’ to 3’, used to amplify and clone estrogen receptors; underlined regions indicate restriction enzyme cut sites.

Degenerate Primers
F	ATGCGGCCGCTAYCAYTAYGGNGTNTGG
R	AGAATTCTRTKNGCRTCNARCATYTC
3’ RACE Primers
Q_T	CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGCT₍₁₇₎VN
Q₀	CCAGTGAGCAGAGTGACG
Q_I	GAGGACTCGAGCTCAAGC
ERα	ACTCCGGAGCCTTTTCCTTC
ERα	ACATTCGCCACATGAGCAAC
ERβ1₀	GCGGTGTGCGAAAAGAGCGTGGGAC
ERβ1_I	CTGGGCCAAGAAGATTCCAG
ERβ2₀	CATTCAACCATGAGCCCAGA
ERβ2_I	ACGCATCATGGATGCTGAAC
GST - Fusion Primers
ERβ1F	TACCATGGATGCTCACATCATGCGCAGCT
ERβ1R	TTCTCGAGCTACTGGGGTTCAGGGTCAC
ERβ2F	TTCCATGGTGTTGGATGCCAACACATCCAGC
ERβ2R	GTCTCGAGTTACCCCTGTAAGGTGGTTTCC