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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Apr 6;1830(9):4314–4320. doi: 10.1016/j.bbagen.2013.03.033

Figure 4. Tumor ECM-induced VEGF secretion by stromal cells stimulates endothelial cell migration.

Figure 4

Media collected from 3T3-L1 cells cultured on tumor ECMs increased HUVEC migration in transwell migration assays relative to media collected from control ECMs, as measured via image analysis of DAPI-stained, fixed membranes following migration. Typical micrographs of migrated HUVECs stained with DAPI are shown at right. Scale bar = 20 μm (A). Blockade of VEGF signaling using a VEGFR2/KDR peptide antagonist confirmed that greater VEGF levels secreted by stromal cells cultured on tumor ECMs contributed to changes in HUVEC migration (B). * indicates p < 0.05.