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. 2013 Jun 20;8(6):e67191. doi: 10.1371/journal.pone.0067191

Figure 3. Regulation of BST2 expression by TGFβ in breast cancer cells.

Figure 3

A. Reduction in baseline BST2 expression detected in 5 grade 1 & 2 cell lines after 24 hr TGFβ (4 ng/ml) treatment. Each QPCR reaction was carried out in triplicate, and values normalized to ACTB expression and to vehicle-only controls. Asterisks represent statistically significant differences (p<0.01) between untreated and TGFβ-treated samples. B. TGFβ-induced shift in AP2 and STAT3 transcript levels under the same treatment conditions employed in panel A. BST2 reduction in grade 1 (CCdl22) & 2 (CCdl66) cell lines is accompanied by a decline in STAT3 levels, while AP2 transcripts increase significantly. No significant changes were observed in grade 3 (CCdl54) cells. Each assay was done in triplicate. Asterisks indicate a significant difference (p<0.01) between untreated and TGFβ-treated samples. C. Inhibition of the TGFβ pathway with 24 hr SB-431542 treatment increased BST2 expression in grade 1 cell lines (CCdl22, CCdl68). Data acquired in triplicate shows significant differences (p<0.01) between test and vehicle-only cell samples, indicated by asterisks.